ENCOREP

From Cell Surface to

Enzymatic-Controlled Release of Proteins (ENCOREP) is an innovative technique that enables the in situ expression and enzymatically controlled release of glycosylated proteins directly at the cell surface.

ENCOREP bypasses the secretory pathway, representing a breakthrough in the production of native, glycosylated proteins for research and therapeutic innovations.This method overcomes limitations of traditional approaches, providing a rapid and reliable way to obtain heavily glycosylated peptides.

An optimized Tobacco Etch Virus (TEV) protease for the ENCOREP system is produced and secreted by the same cells displaying the protein of interest. This enables precise and efficient release of proteins or peptides from the cell surface. The released proteins or peptides can then be collected from the supernatant. 

Why It Matters

Maintain Native Glycosylation Glycosylation is critical for protein folding, stability, solubility, localization, and immunogenicity. ENCOREP captures these native modifications intact—something traditional methods can’t match.

Isolate Difficult Targets

ENCOREP has already succeeded where other approaches have failed, such as isolating highly glycosylated peptides from the large extracellular loop of CD63.

Streamlined Workflow

From in situ expression to protease-triggered release, ENCOREP offers a rapid, reliable, and reproducible pipeline—ideal for research, diagnostics, and therapeutic development.

Potential Applications of the ENCOREP System

Immunization & Vaccine Studies

Expression of antigens with species-specific glycosylation patterns, such as Influenza HA stalk-based antigens, enabling more physiologically relevant immune responses in preclinical models.

Cancer Immunotherapy

Production of glycopeptides for use in tumor-targeted therapies, improving specificity and therapeutic efficacy.

Anti-inflammatory & Anti-thrombotic Therapies

Expression of RGD peptides, known for their role in integrin binding and modulation of inflammation and thrombosis pathways.

Antimicrobial Therapeutics

Generation of Antimicrobial Peptides (AMPs), offering potential alternatives to conventional antibiotics by directly targeting microbial membranes.

Autoimmune Disease Modulation

Expression of peptides derived from MHC Class II extracellular domains, with applications in rebalancing immune tolerance in autoimmune conditions.

Transfect. Express. Collect.

Supernatant, Seamlessly.

Reference

Ivanusic D., Madela K., Burghard H., Holland G., Laue M., Bannert N. tANCHOR: a novel mammalian cell surface peptide display system. Biotechniques. 2021 Jan;70(1):21-28.