Gain of Function Research

Spike Mutations.

Understanding how mutations in the SARS-CoV-2 spike protein affect viral escape and antibody resistance is essential for predicting and preparing for future waves of variants. However, traditional tools are limited by labor-intensive workflows and the need to handle potentially dangerous pathogens, which slows the pace and scope of gain-of-function studies.

The tANCHOR cell-based RBD neutralization assay redefines what's possible.

This next-generation GoF research platform allows researchers to rapidly assess the functional impact of RBD mutations—without the need for recombinant protein expression or ELISA plate coating. Simply introduce spike protein variants, and tANCHOR instantly delivers insights into ACE2 binding dynamics and neutralizing antibody evasion.

Whether you're engineering new spike variants or mapping escape profiles, tANCHOR provides:

• Rapid turnaround for testing multiple mutations in parallel

• Native environment for accurate interaction measurements

• Seamless adaptability to new or hypothetical SARS-CoV-2 variants

tANCHOR: Because understanding tomorrow’s variants starts with better tools today.

Reference

Ivanusic D., Maier J., Icli S., Falcone V., Bernauer H., Bannert N. tANCHOR cell-based assay for SARS- CoV-2 neutralizing antibodies fast adaptive to various receptor-binding domains, iScience, 2024 Feb 5;27(3):109123.

Ivanusic D., Madela K., Burghard H., Holland G., Laue M., Bannert N. tANCHOR: a novel mammalian cell surface peptide display system. Biotechniques. 2021 Jan;70(1):21-28.

Clear Answers.